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worthington 腮腺组织分离指南

发布者:艾美捷科技    发布时间:2022-06-09     
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原代细胞分离中,针对腮腺组织涉及到腮腺和舌下腺,腺泡、腮腺腺泡细胞、上皮,腺泡,眶外泪腺,腮腺,胰腺等等的解离通常会使用酶,如何选择合适的酶来完成对应腮腺组织的解离,以及原代细胞采集的常规操作, 艾美捷针对腮腺相关的组织分类解离分种属、不同的细胞、酶组合推荐,推荐浓度、培养基以及参考文献,详细汇总指南:组织分离指南.png


本技术指南描述了标准实验室程序; 为建立细胞分离方案提供了一种合乎逻辑的实验方法; 并列出了许多组织特异性参考资料,以帮助开发有效的方法。更多其他组织的解离指南,点击查看:各组织分离指南

 

腮腺
种属种属详情细胞名称酶组合推荐,含推荐浓度培养基参考文献
小鼠Mouse腮腺和舌下腺 / Parotid and sublingual glandularNeutral Protease: 50 u/ml;
Collagenase Type 1: 100 u/ml
PBSOgawa, M., Oshima, M., Imamura, A., Sekine, Y., Ishida, K., Yamashita, K., Nakajima, K., Hirayama, M., Tachikawa, T. and Tsuji, T.: Functional Salivary Gland Regeneration by Transplantation of a Bioengineered Organ Germ., Nat Commun 4, 2498, 2013
大鼠Rat, Wistar, male, 130 g腺泡 / AcinarCollagenase: 75 u/ml;
Hyaluronidase: 153 u/ml
RPMI 1640Looms, D., Dissing, S., Tritsaris, K., Pedersen, A. and Nauntofte, B.: Adrenoceptor-Activated Nitric Oxide Synthesis in Salivary Acinar Cells., Adv Dent Res 14, 62, 2000
大鼠Rat, SD, 100-120 g腮腺腺泡细胞 / Parotid acinar cellsCollagenase Type 2: 90 u/mlKrebs-Henseleit BicarbonateD'Silva NJ, DiJulio DH, Belton CM, Jacobson KL, Watson EL: Immunolocalization of Rap1 in the rat parotid gland: detection on secretory granule membranes, J Histochem Cytochem 45, 965-73, 1997
大鼠Rat, SD, male上皮 / EpithelialCollagenase: 50-75 u/ml;
Hyaluronidase: 0.1%
HBSS CFPrasad, K.N., Edwards-Prasad, J., Carvaiho, I., LaRosa, F.G., Baibinder, E., Meyers, A., and Quissell, D.: Establishment of Primary Cultures of Rat and Human Parotid Epithelial Cells for Transfection Experiments, In Vitro Cell Dev Biol 28, 493, 1992
大鼠Rat, SD, male, 100-150 g腮腺腺泡 / Parotid acinarTrypsin: 0.001%F12 mediumYeh, C., Mertz, P., Oliver, C., Baum, B., and Kousvelari, E.: Cellular Characteristics of Long-Term Cultured Rat Parotid Acinar Cells, In Vitro Cell Dev Biol 27, 707, 1991
大鼠Rat, Wistar, male腮腺腺泡 / Parotid acinarTrypsin: 0.02%Solution B (See reference)Foskett, J., Roifman, C. and Wong, D.: Activation of Calcium Oscillations by Thapsigargin in Parotid Acinar Cells, J Biol Chem 266, 2778, 1991
大鼠Rat腮腺 / ParotidHyaluronidase: 0.025%HBSS with 20mM HEPESTakuma, T. and Ichida, T.: Amylase Secretion From Saponin-Permeabilized Parotid Cells Evoked by Cyclic AMP, J Invest Dermatol 103, 95, 1988
大鼠Rat, Wistar, male, 250 - 350 g腺泡,腮腺 / Acinar, parotidHyaluronidase: 0.015%Earle's MEMMelvin, J., Kawaguchi, M., Baum, B., and Turner, R.: A Muscarinic Agonist-Stimulated Chloride Efflux Pathway is Associated With Fluid Secretion in Rat Parotid Acinar Cells, Biochem Biophys Res Commun 145, 754, 1987
大鼠Rat, SD, male, 50 - 125 g腺泡,眶外泪腺,腮腺,胰腺 / Acinar,Exorbital lacrimal, parotid, pancreasTrypsin: 0.01%HBSS, CMFOliver, C., Waters, J., Tolbert, C., and Kleinman, H.: Growth of Exocrine Acinar Cells on a Reconstituted Basement Membrane Gel, In Vitro Cell Dev Biol 23, 465, 1987
大鼠Rat, SD, albino, male, 150-200 g腺泡,腮腺 / Acinar, parotidTrypsin: 0.01%;
Collagenase: 40-50 u/ml;
Hyaluronidase: 0.10%
HBSS CMFMangos, J., McSherry, N., Butcher, F., Irwin, K., and Barber, T.: Dispersed Rat Parotid Acinar Cells (Morphological, Functional Character), Am J Physiol 229 (3), 560, 1975


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